hcr probe hybridization buffer Search Results


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( A ) Representative images of bmp4 -injected zebrafish embryos (upper) and explants (lower, with injection site towards the right) from 2.25 to 24 hpf. Red fluorescence marks the descendants of the bmp4 -injected blastomere. Arrowheads at 8 hpf and 10 hpf embryo point to the protrusions induced by Bmp4 in embryos, and at 24 hpf in explants to the segmented somites. Labels indicate the primary (i) and secondary axis (ii). ( B ) Immunofluorescence imaging of Phospho-Smad1/5/9 in bmp4 + RFP injected explant (upper) and RFP injected explant (lower) at 4 hpf, with the injection site towards the right. Signal measurements along the dotted rectangles are shown on the right. Dark Green curves represent the nonlinear fit of actual data curves (light green), with Dark Green numbers indicating the mean slopes. Red curves depict the distributions of RFP signaling intensity along the dotted rectangles. ( C ) Quantification of the elongation of Bmp4 explants from 4 to 24 hpf. The Midlines along anterior–posterior axis of Bmp4 explants are measured (red line), as displayed in the upper left. Data are presented as mean ± SD. ( D ) bmp4 -injected explants (upper, posterior towards the right) and embryos (lower, animal pole view) at 6 hpf stained by whole mount in situ hybridization (WISH) with the indicated probes. DAB staining of GFP (brown spots) indicates the clones of bmp4 & GFP mRNA injected blastomere at 2.25 hpf. ( E ) Bmp4 explants at 14 hpf stained by WISH with indicated probes. ( F ) Whole mount in situ <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> staining of Bmp4 explants at 12 hpf for tbxta and sox2 , with co-staining of DAPI. NT neural tube, MP neuromesodermal progenitor, TMP tailbud mesodermal progenitor. ( G ) Time-lapse imaging of Bmp4 explants displaying the sequential formation of somites, as per Movie . White dotted lines indicate existing somites, and purple dotted lines indicate newly formed somites. Nuclei were labeled by H2B-GFP (green fluorescence). ( H ) Bmp4 explants at 24 hpf stained by WISH with the indicated probes. For all explants displayed in this article, anterior (opposite to the injection site) is toward the top unless otherwise stated. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 μm. .
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( A ) Representative images of bmp4 -injected zebrafish embryos (upper) and explants (lower, with injection site towards the right) from 2.25 to 24 hpf. Red fluorescence marks the descendants of the bmp4 -injected blastomere. Arrowheads at 8 hpf and 10 hpf embryo point to the protrusions induced by Bmp4 in embryos, and at 24 hpf in explants to the segmented somites. Labels indicate the primary (i) and secondary axis (ii). ( B ) Immunofluorescence imaging of Phospho-Smad1/5/9 in bmp4 + RFP injected explant (upper) and RFP injected explant (lower) at 4 hpf, with the injection site towards the right. Signal measurements along the dotted rectangles are shown on the right. Dark Green curves represent the nonlinear fit of actual data curves (light green), with Dark Green numbers indicating the mean slopes. Red curves depict the distributions of RFP signaling intensity along the dotted rectangles. ( C ) Quantification of the elongation of Bmp4 explants from 4 to 24 hpf. The Midlines along anterior–posterior axis of Bmp4 explants are measured (red line), as displayed in the upper left. Data are presented as mean ± SD. ( D ) bmp4 -injected explants (upper, posterior towards the right) and embryos (lower, animal pole view) at 6 hpf stained by whole mount in situ hybridization (WISH) with the indicated probes. DAB staining of GFP (brown spots) indicates the clones of bmp4 & GFP mRNA injected blastomere at 2.25 hpf. ( E ) Bmp4 explants at 14 hpf stained by WISH with indicated probes. ( F ) Whole mount in situ <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> staining of Bmp4 explants at 12 hpf for tbxta and sox2 , with co-staining of DAPI. NT neural tube, MP neuromesodermal progenitor, TMP tailbud mesodermal progenitor. ( G ) Time-lapse imaging of Bmp4 explants displaying the sequential formation of somites, as per Movie . White dotted lines indicate existing somites, and purple dotted lines indicate newly formed somites. Nuclei were labeled by H2B-GFP (green fluorescence). ( H ) Bmp4 explants at 24 hpf stained by WISH with the indicated probes. For all explants displayed in this article, anterior (opposite to the injection site) is toward the top unless otherwise stated. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 μm. .
Hcr V3 0 Probe Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Representative images of bmp4 -injected zebrafish embryos (upper) and explants (lower, with injection site towards the right) from 2.25 to 24 hpf. Red fluorescence marks the descendants of the bmp4 -injected blastomere. Arrowheads at 8 hpf and 10 hpf embryo point to the protrusions induced by Bmp4 in embryos, and at 24 hpf in explants to the segmented somites. Labels indicate the primary (i) and secondary axis (ii). ( B ) Immunofluorescence imaging of Phospho-Smad1/5/9 in bmp4 + RFP injected explant (upper) and RFP injected explant (lower) at 4 hpf, with the injection site towards the right. Signal measurements along the dotted rectangles are shown on the right. Dark Green curves represent the nonlinear fit of actual data curves (light green), with Dark Green numbers indicating the mean slopes. Red curves depict the distributions of RFP signaling intensity along the dotted rectangles. ( C ) Quantification of the elongation of Bmp4 explants from 4 to 24 hpf. The Midlines along anterior–posterior axis of Bmp4 explants are measured (red line), as displayed in the upper left. Data are presented as mean ± SD. ( D ) bmp4 -injected explants (upper, posterior towards the right) and embryos (lower, animal pole view) at 6 hpf stained by whole mount in situ hybridization (WISH) with the indicated probes. DAB staining of GFP (brown spots) indicates the clones of bmp4 & GFP mRNA injected blastomere at 2.25 hpf. ( E ) Bmp4 explants at 14 hpf stained by WISH with indicated probes. ( F ) Whole mount in situ <t>hybridization</t> <t>chain</t> <t>reaction</t> <t>(HCR)</t> staining of Bmp4 explants at 12 hpf for tbxta and sox2 , with co-staining of DAPI. NT neural tube, MP neuromesodermal progenitor, TMP tailbud mesodermal progenitor. ( G ) Time-lapse imaging of Bmp4 explants displaying the sequential formation of somites, as per Movie . White dotted lines indicate existing somites, and purple dotted lines indicate newly formed somites. Nuclei were labeled by H2B-GFP (green fluorescence). ( H ) Bmp4 explants at 24 hpf stained by WISH with the indicated probes. For all explants displayed in this article, anterior (opposite to the injection site) is toward the top unless otherwise stated. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 μm. .
E Spl Mγ Hcr Probe Sets Molecular Instruments, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Representative images of bmp4 -injected zebrafish embryos (upper) and explants (lower, with injection site towards the right) from 2.25 to 24 hpf. Red fluorescence marks the descendants of the bmp4 -injected blastomere. Arrowheads at 8 hpf and 10 hpf embryo point to the protrusions induced by Bmp4 in embryos, and at 24 hpf in explants to the segmented somites. Labels indicate the primary (i) and secondary axis (ii). ( B ) Immunofluorescence imaging of Phospho-Smad1/5/9 in bmp4 + RFP injected explant (upper) and RFP injected explant (lower) at 4 hpf, with the injection site towards the right. Signal measurements along the dotted rectangles are shown on the right. Dark Green curves represent the nonlinear fit of actual data curves (light green), with Dark Green numbers indicating the mean slopes. Red curves depict the distributions of RFP signaling intensity along the dotted rectangles. ( C ) Quantification of the elongation of Bmp4 explants from 4 to 24 hpf. The Midlines along anterior–posterior axis of Bmp4 explants are measured (red line), as displayed in the upper left. Data are presented as mean ± SD. ( D ) bmp4 -injected explants (upper, posterior towards the right) and embryos (lower, animal pole view) at 6 hpf stained by whole mount in situ hybridization (WISH) with the indicated probes. DAB staining of GFP (brown spots) indicates the clones of bmp4 & GFP mRNA injected blastomere at 2.25 hpf. ( E ) Bmp4 explants at 14 hpf stained by WISH with indicated probes. ( F ) Whole mount in situ hybridization chain reaction (HCR) staining of Bmp4 explants at 12 hpf for tbxta and sox2 , with co-staining of DAPI. NT neural tube, MP neuromesodermal progenitor, TMP tailbud mesodermal progenitor. ( G ) Time-lapse imaging of Bmp4 explants displaying the sequential formation of somites, as per Movie . White dotted lines indicate existing somites, and purple dotted lines indicate newly formed somites. Nuclei were labeled by H2B-GFP (green fluorescence). ( H ) Bmp4 explants at 24 hpf stained by WISH with the indicated probes. For all explants displayed in this article, anterior (opposite to the injection site) is toward the top unless otherwise stated. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 μm. .

Journal: The EMBO Journal

Article Title: BMP4 initiates and patterns ventral-caudal structures in zebrafish and human pluripotent stem cell aggregates

doi: 10.1038/s44318-025-00643-6

Figure Lengend Snippet: ( A ) Representative images of bmp4 -injected zebrafish embryos (upper) and explants (lower, with injection site towards the right) from 2.25 to 24 hpf. Red fluorescence marks the descendants of the bmp4 -injected blastomere. Arrowheads at 8 hpf and 10 hpf embryo point to the protrusions induced by Bmp4 in embryos, and at 24 hpf in explants to the segmented somites. Labels indicate the primary (i) and secondary axis (ii). ( B ) Immunofluorescence imaging of Phospho-Smad1/5/9 in bmp4 + RFP injected explant (upper) and RFP injected explant (lower) at 4 hpf, with the injection site towards the right. Signal measurements along the dotted rectangles are shown on the right. Dark Green curves represent the nonlinear fit of actual data curves (light green), with Dark Green numbers indicating the mean slopes. Red curves depict the distributions of RFP signaling intensity along the dotted rectangles. ( C ) Quantification of the elongation of Bmp4 explants from 4 to 24 hpf. The Midlines along anterior–posterior axis of Bmp4 explants are measured (red line), as displayed in the upper left. Data are presented as mean ± SD. ( D ) bmp4 -injected explants (upper, posterior towards the right) and embryos (lower, animal pole view) at 6 hpf stained by whole mount in situ hybridization (WISH) with the indicated probes. DAB staining of GFP (brown spots) indicates the clones of bmp4 & GFP mRNA injected blastomere at 2.25 hpf. ( E ) Bmp4 explants at 14 hpf stained by WISH with indicated probes. ( F ) Whole mount in situ hybridization chain reaction (HCR) staining of Bmp4 explants at 12 hpf for tbxta and sox2 , with co-staining of DAPI. NT neural tube, MP neuromesodermal progenitor, TMP tailbud mesodermal progenitor. ( G ) Time-lapse imaging of Bmp4 explants displaying the sequential formation of somites, as per Movie . White dotted lines indicate existing somites, and purple dotted lines indicate newly formed somites. Nuclei were labeled by H2B-GFP (green fluorescence). ( H ) Bmp4 explants at 24 hpf stained by WISH with the indicated probes. For all explants displayed in this article, anterior (opposite to the injection site) is toward the top unless otherwise stated. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 μm. .

Article Snippet: HCR probe wash buffer , Molecular Instruments , BPW02424.

Techniques: Injection, Fluorescence, Immunofluorescence, Imaging, Staining, In Situ Hybridization, Clone Assay, Labeling